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KMID : 0545120090190121603
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Journal of Microbiology and Biotechnology
2009 Volume.19 No. 12 p.1603 ~ p.1611
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High-Cell-Density Fed-Batch Culture of Saccharomyces cerevisiae KV-25 Using Molasses and Corn Steep Liquor
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Vu Van Hanh
Kim Keun
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Abstract
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In the large ethanol production plants, seeding culture usually takes volume of 4-10% (v/v) of working culture. It is necessary to reduce the cost of seeding culture by using high cell density cultivation together with using low value and available materials. The production of cell mass of Saccharomyces cerevisiae KV-25 was significantly depended on the ratio of C- and N-source. The concentrations of molasses and corn steep liquor (CSL) in the culture medium were statistically optimized to be 10.25%(v/v) and 16.87%(v/v) by response surface methodology. The optimized medium used for batch culture in a 5 l stirred tank reactor produced a cell mass production of 36.5 g/l. The feed phase was preceded by a batch phase with the optimized medium, and very high cell mass yield of 187.63 g dried cell/l was successfully accomplished by feeding a mixture of molasses 20% (v/v) and 80% (v/v) of CSL at a feed rate of 22 ml/h. In fed-batch culture with the stirred tank reactor, the production of cell mass was depended mainly on agitation speed, feed medium and glucose level in the medium but slightly on aeration rate.
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KEYWORD
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High-cell-density culture, Saccharomyces cerevisiae, molasses, corn steep liquor, fed-batch, response surface methodology
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